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Rolling Circle Amplification With Hairpin Probes for In Situ Single-Molecule RNA Imaging at Single-Base Resolution.

Chemistry (Weinheim an der Bergstrasse, Germany) - 2 Jul 2026

Yue Chensirong, Tu Changyun, Liu Yang, Zhang Yuanru, Yang Chaoyong, Liu Dan

Abstract excerpt

We present an in situ single-molecule RNA imaging method, termed hairpin-primed rolling circle amplification (RCA) imaging assay (HPRIA), which achieves single-base resolution and subcellular localization. This method utilizes the metastable stem-loop structure of hairpin probes, which unfolds only when perfectly complementary to the target RNA, thereby enabling the imaging of RNA molecules with single-nucleotide...

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PMID: 41999069
DOI: 10.1002/chem.70999

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