Article
Novel upstream and intragenic control elements for the RNA polymerase III-dependent transcription of human 7SL RNA genes.
Biochimie - 1 Dec 2004
Englert Markus, Felis Martha, Junker Volker, Beier Hildburg
Abstract excerpt
In the human nuclear genome only a few copies coding for full-length 7SL RNA genes exist. The Hs7SL-1 gene has recently been classified as type 4 of RNA polymerase III (pol III)-transcribed genes as it was demonstrated that mutations in an external transcriptional activator (ATF) binding site and in an internal CG dinucleotide at positions +15/+16 reduced 7SL RNA expression in vivo and in vitro. We have extended...
Topics
- 5' Flanking Region
- Base Sequence
- Binding Sites
- Cell Extracts
- Chromosomes, Human, Pair 14
- Cloning, Molecular
- Conserved Sequence
- DNA Mutational Analysis
- DNA, Complementary
- Databases, Genetic
- Gene Amplification