Article
Expression of a human 7S K RNA gene in vivo requires a novel pol III upstream element.
The EMBO journal - 1 Mar 1990
Kleinert H, Bredow S, Benecke B J
Abstract excerpt
The 5'-flanking sequences required for expression of a human 7S K RNA gene have been defined by mutant analysis. A -111 upstream deletion mutant showed full activity when analysed by in vitro transcription with HeLa cell extracts. In contrast, upon transfection into intact cells, this mutant only...
Topics
- Base Sequence
- Chromosome Deletion
- Cloning, Molecular
- DNA-Directed RNA Polymerases
- Gene Expression Regulation
- HeLa Cells
- Humans
- Molecular Sequence Data
- Mutation
- Promoter Regions, Genetic
- RNA Polymerase III
- Restriction Mapping
- Ribonucleoproteins
- Ribonucleoproteins, Small Nuclear
- Transcription, Genetic
- Transfection