The human U1 snRNA promoter correctly initiates transcription in vitro and is activated by PSE1.

doi:10.1101/gad.4.12a.2048

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Article

The human U1 snRNA promoter correctly initiates transcription in vitro and is activated by PSE1.

Genes & development - 1 Dec 1990

Gunderson S I, Knuth M W, Burgess R R

Abstract excerpt

A DNA-dependent in vitro transcription system for the human U1 small nuclear RNA (snRNA) promoter has been developed. This in vitro transcription system uses extracts of tissue culture cells to drive transcription of an RNA polymerase II-transcribed snRNA gene. A U1 promoter (-393 to +192) templa...

Topics

Base Sequence
Cloning, Molecular
DNA-Binding Proteins
Guanosine Triphosphate
HeLa Cells
Humans
Molecular Sequence Data
Mutation
Promoter Regions, Genetic
Proteins
RNA Polymerase II
RNA, Small Nuclear
Ribonuclease T1
Templates, Genetic
Transcription, Genetic

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