Article
The human U1 snRNA promoter correctly initiates transcription in vitro and is activated by PSE1.
Genes & development - 1 Dec 1990
Gunderson S I, Knuth M W, Burgess R R
Abstract excerpt
A DNA-dependent in vitro transcription system for the human U1 small nuclear RNA (snRNA) promoter has been developed. This in vitro transcription system uses extracts of tissue culture cells to drive transcription of an RNA polymerase II-transcribed snRNA gene. A U1 promoter (-393 to +192) template was constructed in which the sequences from +10 to +171 were replaced with a 179-bp sequence from a G-less cassette....
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