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Article

Sequence and factor requirements for faithful in vitro transcription of human 7SL DNA.

Gene - 14 Feb 1990

Bredow S, Kleinert H, Benecke B J

Abstract excerpt

We have analysed the transcription of a functional human 7SL gene by RNA polymerase III (RNAPIII) in S100 extracts in vitro. Accurate and efficient synthesis of 7S L RNA depends on the presence of (i) an upstream sequence and (ii) an internal promoter element located within the first 22 bp of the gene. These findings were substantiated by DNase I footprinting. Mutations of the internal promoter identified the...

Topics

Base Sequence
DNA
Deoxyribonuclease I
Genes
Humans
Introns
Molecular Sequence Data
Molecular Weight
Mutation
Nucleotide Mapping
Promoter Regions, Genetic
RNA Polymerase III

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Templates, Genetic

Transcription, Genetic

Source

Identifiers

PMID: 2323574
DOI: 10.1016/0378-1119(90)90282-v

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-2323574-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

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