Maturation of bacteriophage T4 lagging strand fragments depends on interaction of T4 RNase H with T4 32 protein rather than the T4 gene 45 clamp.

Abstract excerpt

In the bacteriophage T4 DNA replication system, T4 RNase H removes the RNA primers and some adjacent DNA before the lagging strand fragments are ligated. This 5'-nuclease has strong structural and functional similarity to the FEN1 nuclease family. We have shown previously that T4 32 protein binds DNA behind the nuclease and increases its processivity. Here we show that T4 RNase H with a C-terminal deletion...

Topics

• Amino Acid Sequence
• Bacteriophage T4
• Binding Sites
• Catalytic Domain
• Crystallography, X-Ray
• DNA
• DNA Primers
• Gene Deletion
• Genetic Complementation Test
• Humans
• Models, Genetic
• Models, Molecular
• Molecular Sequence Data
• Mutation