Article
Role of nucleotidyl transferase motif V in strand joining by chlorella virus DNA ligase.
The Journal of biological chemistry - 22 Mar 2002
Sriskanda Verl, Shuman Stewart
Abstract excerpt
ATP-dependent DNA ligases, NAD(+)-dependent DNA ligases, and GTP-dependent RNA capping enzymes are members of a covalent nucleotidyl transferase superfamily defined by a common fold and a set of conserved peptide motifs. Here we examined the role of nucleotidyl transferase motif V ((184)LLKMKQFKDAEAT(196)) in the nick joining reaction of Chlorella virus DNA ligase, an exemplary ATP-dependent enzyme. We found that...
Topics
- Adenosine Triphosphate
- Alanine
- Amino Acid Motifs
- Amino Acid Sequence
- Aspartic Acid
- DNA
- DNA Ligases
- DNA Mutational Analysis
- DNA Nucleotidyltransferases
- Dose-Response Relationship, Drug
- Electrophoresis, Polyacrylamide Gel
- Glutamine
- Kinetics
- Lysine
- Molecular Sequence Data
- Mutation
- Mutation, Missense
- Point Mutation