Article
Structural and functional characterization of deep-sea thermophilic bacteriophage GVE2 HNH endonuclease.
Scientific reports - 13 Feb 2017
Zhang Likui, Xu Dandan, Huang Yanchao, Zhu Xinyuan, Rui Mianwen, Wan Ting, Zheng Xin, Shen Yulong, Chen Xiangdong, Ma Kesen, Gong Yong
Abstract excerpt
HNH endonucleases in bacteriophages play a variety of roles in the phage lifecycle as key components of phage DNA packaging machines. The deep-sea thermophilic bacteriophage Geobacillus virus E2 (GVE2) encodes an HNH endonuclease (GVE2 HNHE). Here, the crystal structure of GVE2 HNHE is reported. This is the first structural study of a thermostable HNH endonuclease from a thermophilic bacteriophage. Structural...
Topics
- Amino Acid Sequence
- Bacteriophages
- Catalytic Domain
- Endonucleases
- Manganese
- Models, Molecular
- Mutation
- Protein Binding
- Protein Conformation
- Protein Interaction Domains and Motifs
- Protein Stability
- Structure-Activity Relationship
- Thermodynamics