Article
C-terminal phenylalanine of bacteriophage T7 single-stranded DNA-binding protein is essential for strand displacement synthesis by T7 DNA polymerase at a nick in DNA.
The Journal of biological chemistry - 30 Oct 2009
Ghosh Sharmistha, Marintcheva Boriana, Takahashi Masateru, Richardson Charles C
Abstract excerpt
Single-stranded DNA-binding protein (gp2.5), encoded by gene 2.5 of bacteriophage T7, plays an essential role in DNA replication. Not only does it remove impediments of secondary structure in the DNA, it also modulates the activities of the other replication proteins. The acidic C-terminal tail of gp2.5, bearing a C-terminal phenylalanine, physically and functionally interacts with the helicase and DNA...
Topics
- Amino Acid Substitution
- Bacteriophage T7
- DNA, Single-Stranded
- DNA-Binding Proteins
- DNA-Directed DNA Polymerase
- Mutation
- Phenylalanine
- Protein Binding
- Repressor Proteins