Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

Processing of A-form ssDNA by cryptic RNase H fold exonuclease PF2046.

Archives of biochemistry and biophysics - 15 Sept 2016

Kim Junsoo, Sambalkhundev Gerelt-Od, Kim Sulhee, Son Jonghyeon, Han Ah-Reum, Ko Sul-Min, Hwang Kwang Yeon, Lee Woo Cheol

Abstract excerpt

RNase H fold protein PF2046 of Pyrococcus furiosus is a 3'-5' ssDNA exonuclease that cleaves after the second nucleotide from the 3' end of ssDNA and prefers poly-dT over poly-dA as a substrate. In our crystal structure of PF2046 complexed with an oligonucleotide of four thymidine nucleotides (dT4), PF2046 accommodates dT4 tightly in a groove and imposes steric hindrance on dT4 mainly by Phe220 such that dT4...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to Catalytic Domain
Share to Crystallization

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Share to DNA, Single-Stranded

Share to Exonucleases

Share to Oligodeoxyribonucleotides

Share to Pyrococcus furiosus

Share to Ribonuclease H

Share to X-Ray Diffraction

Source

Identifiers

PMID: 27495739
DOI: 10.1016/j.abb.2016.08.001

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

Processing of A-form ssDNA by cryptic RNase H fold exonuclease PF2046.PMID 27495739Read ↗

Structural basis for substrate recognition and processive cleavage mechanisms of the trimeric exonuclease PhoExo I.Semantic proximity Read ↗

Functional interdependence of DNA polymerizing and 3'-->5' exonucleolytic activities in Pyrococcus furiosus DNA polymerase I.Semantic proximity Read ↗

DNA interference by a mesophilic Argonaute protein, CbcAgoSemantic proximity Read ↗

Aromatic residues located close to the active center are essential for the catalytic reaction of flap endonuclease-1 from hyperthermophilic archaeon Pyrococcus horikoshii.Semantic proximity Read ↗

The closed structure of an archaeal DNA ligase from Pyrococcus furiosus.Semantic proximity Read ↗

Crystal structure of Caulobacter crescentus polynucleotide phosphorylase reveals a mechanism of RNA substrate channelling and RNA degradosome assembly.Semantic proximity Read ↗

Phage-encoded factor stimulates DNA degradation by the Hna anti-phage defense system.Semantic proximity Read ↗

Mutations to create thermostable reverse transcriptase with bacterial family A DNA polymerase from Thermotoga petrophila K4.Semantic proximity Read ↗

The stepwise endonuclease activity of a thermophilic Argonaute proteinSemantic proximity Read ↗

Increased Processivity, Misincorporation, and Nucleotide Incorporation Efficiency in Sulfolobus solfataricus Dpo4 Thumb Domain Mutants.Semantic proximity Read ↗

Novel ribonucleotide discrimination in the RNA polymerase-like two-barrel catalytic core of Family D DNA polymerases.Semantic proximity Read ↗

Structural insights into RNA unwinding and degradation by RNase R.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.