Article
Characterization of a mutant Listeria monocytogenes strain expressing green fluorescent protein.
Acta biochimica et biophysica Sinica - 1 Jan 2005
Jiang Ling-Li, Song Hou-Hui, Chen Xue-Yan, Ke Chun-Lin, Xu Jing-Jing, Chen Ning, Fang Wei-Huan
Abstract excerpt
To construct a recombinant strain of Listeria monocytogenes for the expression of heterologous genes, homologous recombination was utilized for insertional mutation, targeting its listeriolysin O gene (hly). The gene encoding green fluorescent protein (GFP) was used as the indicator of heterologous gene expression. The gene gfp was inserted into hly downstream from its promoter and signal sequence by an...
Read the complete abstract on PubMedTopics
Share this publication in a Topic to start or enrich a Post.
