Article
Insertional mutagenesis of Listeria monocytogenes with a novel Tn917 derivative that allows direct cloning of DNA flanking transposon insertions.
Journal of bacteriology - 1 Jul 1990
Camilli A, Portnoy A, Youngman P
Abstract excerpt
To carry out efficient insertional mutagenesis in Listeria monocytogenes and to facilitate the characterization of disrupted genes, two novel derivatives of Tn917 were constructed, Tn917-LTV1 and Tn917-LTV3. The derivatives (i) transpose at a significantly elevated frequency, (ii) generate transcriptional lacZ fusions when inserted into a chromosomal gene in the appropriate orientation, and (iii) allow the rapid...
Topics
- Bacillus subtilis
- Chromosomes, Bacterial
- Cloning, Molecular
- DNA Transposable Elements
- DNA, Bacterial
- Drug Resistance, Microbial
- Listeria monocytogenes
- Mutation
- Plasmids
- Restriction Mapping
- Transformation, Bacterial