Article
Tools for functional postgenomic analysis of listeria monocytogenes.
Applied and environmental microbiology - 1 Jul 2008
Monk Ian R, Gahan Cormac G M, Hill Colin
Abstract excerpt
We describe the development of genetic tools for regulated gene expression, the introduction of chromosomal mutations, and improved plasmid transfer by electroporation in the food-borne pathogen Listeria monocytogenes. pIMK, a kanamycin-resistant, site-specific, integrative listeriophage vector was constructed and then modified for overexpression (pIMK2) or for isopropyl-beta-d-thiogalactopyranoside...
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