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Article

An improved cloning vector for construction of gene replacements in Listeria monocytogenes.

Applied and environmental microbiology - 1 May 2003

Li Guojie, Kathariou S

Abstract excerpt

Listeria monocytogenes is a gram-positive, facultative intracellular bacterium implicated in severe food-borne illness (listeriosis) in humans. The construction of well-defined gene replacements in the genome of L. monocytogenes has been instrumental to several genetic studies of the virulence and other attributes of the organism. Construction of such mutations by currently available procedures, however, tends to...

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Identifiers

PMID: 12732583
DOI: 10.1128/AEM.69.5.3020-3023.2003
PMCID: PMC154530

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Source receipt: pubmed, retrieved 7 Sept 2026.

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