Selection and analysis of galactose metabolic pathway variants of a mouse liver cell line.

doi:10.1128/mcb.10.9.4582-4589.1990

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Article

Selection and analysis of galactose metabolic pathway variants of a mouse liver cell line.

Molecular and cellular biology - 1 Sept 1990

Zaret K S, Stevens K A

Abstract excerpt

To study the genetic expression and regulation of galactose-metabolizing enzymes, we mutagenized the mouse liver H2.35 cell line and selected for cell clones resistant to the toxic galactose analog, 2-deoxy-D-galactose (2-DOG). One cloned line, designated H12.10, was stably resistant to high leve...

Topics

Animals
Base Sequence
Cell Line
Clone Cells
Escherichia coli
Galactokinase
Galactose
Genes, Bacterial
Genetic Variation
Liver
Mice
Molecular Sequence Data
Nucleotidyltransferases
Oligonucleotide Probes
Transfection
UDPglucose-Hexose-1-Phosphate Uridylyltransferase

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