Article
A real-time PCR approach for rapid high resolution subtyping of HLA-DRB1*04.
Journal of immunological methods - 20 Dec 2006
Gersuk Vivian H, Nepom Gerald T
Abstract excerpt
We present a real-time PCR approach for the identification and subtyping of HLA-DR4 alleles. The technique, which uses sequence-specific primers and probes in conjunction with real-time PCR for the detection and differentiation of target alleles, is rapid, involves minimal hands-on time, and is inexpensive compared to existing methods. Further, there is no post-PCR handling, so the risk of contamination is...
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