Article
Rapid HLA-DR fluorotyping based on melting curve analysis.
Immunological investigations - 1 Jan 2007
Liu Yang, Zhu Qiyun, Zhu Naishuo
Abstract excerpt
Real-time polymerase chain reaction (PCR) has been used in the study of human leukocyte antigen (HLA) genotyping as a potential alternative for routinely used molecular methods such as PCR-sequence specific primers (PCR-SSP) and PCR-sequence specific oligonucleotide probes (PCR-SSO). Combined with fluorescent dye like SYBR GREEN I, it has more advantages such as low cost and consistent background. The aim of this...
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