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Article

Real-time detection of basal and stimulated G protein GTPase activity using fluorescent GTP analogues.

The Journal of biological chemistry - 4 Mar 2005

Jameson Emily E, Roof Rebecca A, Whorton Matthew R, Mosberg Henry I, Sunahara Roger K, Neubig Richard R, Kennedy Robert T

Abstract excerpt

Hydrolysis of fluorescent GTP analogues BODIPY FL guanosine 5 '-O-(thiotriphosphate) (BGTPgammaS) and BODIPY FL GTP (BGTP) by Galpha(i1) and Galpha was characterized using on-line capillary electrophoresis (o) laser-induced fluorescence assays in order that changes in sub-strate, substrate-enzyme complex, and product could be monitored separately. Apparent k values (V /[E]) (max cat) steady-state and K(m) values...

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PMID: 15613467
DOI: 10.1074/jbc.M413810200

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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