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Article

Internally ratiometric fluorescent sensors for evaluation of intracellular GTP levels and distribution.

Nature methods - 1 Oct 2017

Bianchi-Smiraglia Anna, Rana Mitra S, Foley Colleen E, Paul Leslie M, Lipchick Brittany C, Moparthy Sudha, Moparthy Kalyana, Fink Emily E, Bagati Archis, Hurley Edward, Affronti Hayley C, Bakin Andrei V, Kandel Eugene S, Smiraglia Dominic J, Feltri Maria Laura, Sousa Rui, Nikiforov Mikhail A

Abstract excerpt

GTP is a major regulator of multiple cellular processes, but tools for quantitative evaluation of GTP levels in live cells have not been available. We report the development and characterization of genetically encoded GTP sensors, which we constructed by inserting a circularly permuted yellow fluorescent protein (cpYFP) into a region of the bacterial G protein FeoB that undergoes a GTP-driven conformational...

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PMID: 28869758
DOI: 10.1038/nmeth.4404
PMCID: PMC5636219

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