Article

HPLC Method to Resolve, Identify and Quantify Guanine Nucleotides Bound to the GTPase Ras

2021-06-29

Abstract excerpt

The Ras superfamily of small G proteins play central roles in diverse signaling pathways. Superfamily members act as molecular on-off switches defined by their occupancy with GTP or GDP, respectively. In vitro functional studies require loading with a hydrolysis-resistant GTP analogue to increase the on-state lifetime, as well as knowledge of fractional loading with activating and inactivating nucleotides. The pr...

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Literature Corpus work: 5915c2f5-1e7c-5fe9-ae5f-16b8685143aa
DOI: 10.1101/2021.06.28.450218

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