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Article

HPLC method to resolve, identify and quantify guanine nucleotides bound to recombinant ras GTPase.

Analytical biochemistry - 15 Oct 2021

Hannan Jonathan P, Swisher G Hayden, Martyr Justin G, Cordaro Nicholas J, Erbse Annette H, Falke Joseph J

Abstract excerpt

The Ras superfamily of small G proteins play central roles in diverse signaling pathways. Superfamily members act as molecular on-off switches defined by their occupancy with GTP or GDP, respectively. In vitro functional studies require loading with a hydrolysis-resistant GTP analogue to increase the on-state lifetime, as well as knowledge of fractional loading with activating and inactivating nucleotides. The...

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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Identifiers

PMID: 34433016
DOI: 10.1016/j.ab.2021.114338
PMCID: PMC8511091

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