Cloning and nucleotide sequence determination of twelve mutant dnaA genes of Escherichia coli.

doi:10.1007/BF00272340

Back to search

Article

Cloning and nucleotide sequence determination of twelve mutant dnaA genes of Escherichia coli.

Molecular & general genetics : MGG - 1 Jul 1992

Hansen F G, Koefoed S, Atlung T

Abstract excerpt

Plasmids carrying different regions of the wild-type dnaA gene were used for marker rescue analysis of the temperature sensitivity of twelve strains carrying dnaA mutations. The different dnaA(Ts) mutations could be unambiguously located within specific regions of the dnaA gene. The mutant dnaA g...

Topics

Alanine
Bacterial Proteins
Base Sequence
Cloning, Molecular
DNA-Binding Proteins
Escherichia coli
Genes, Bacterial
Genetic Complementation Test
Mutation
Phenotype
Plasmids
Valine

Community interpretation

Discuss the evidence

Ask a question, add context or challenge an interpretation. Community activity is separate from scientific evidence strength.

discussions

replies

Citations encouraged

Claims remain attributable.

No discussion yet

Be the first to add a careful, cited interpretation.