Article
Cloning and nucleotide sequence determination of twelve mutant dnaA genes of Escherichia coli.
Molecular & general genetics : MGG - 1 Jul 1992
Hansen F G, Koefoed S, Atlung T
Abstract excerpt
Plasmids carrying different regions of the wild-type dnaA gene were used for marker rescue analysis of the temperature sensitivity of twelve strains carrying dnaA mutations. The different dnaA(Ts) mutations could be unambiguously located within specific regions of the dnaA gene. The mutant dnaA genes were cloned on pBR322-derived plasmids and on nucleotide sequencing by dideoxy chain termination the respective...
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