Article
Novel alleles of the Escherichia coli dnaA gene.
Journal of molecular biology - 5 Sept 1997
Sutton M D, Kaguni J M
Abstract excerpt
The Escherichia coli dnaA gene is required for replication of the bacterial chromosome. To identify residues critical for its replication activity, a method to select novel mutations was developed that relied on lytic growth of lambda from an inserted pSC101 replication origin. Replication from the lambda origin was inhibited by lysogen-encoded cI repressor. Replication from the pSC101 origin that resulted in...
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