Article
Somatically mutated IgG anti-DNA antibody clonally related to germ-line encoded IgM anti-DNA antibody.
European journal of immunology - 1 Apr 1992
Taki S, Hirose S, Kinoshita K, Nishimura H, Shimamura T, Hamuro J, Shirai T
Abstract excerpt
Among 15 anti-DNA antibody-producing hybridomas derived from a single NZB X NZW F1 mouse, an IgM and an IgG were shown to use the same VH gene of the Q52 family. Using a combination of two primers both consisting of a mixture of oligonucleotides (one complementary to the 5' end of VH segment and one to the 3' end of VH segment of Q52 family) we determined the sequences of several members of germ-line VH genes in...
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