Article
Somatically mutated IgG anti-DNA antibody clonally related to germ-line encoded IgM anti-DNA antibody.
European journal of immunology - 1 Apr 1992
Taki S, Hirose S, Kinoshita K, Nishimura H, Shimamura T, Hamuro J, Shirai T
Abstract excerpt
Among 15 anti-DNA antibody-producing hybridomas derived from a single NZB X NZW F1 mouse, an IgM and an IgG were shown to use the same VH gene of the Q52 family. Using a combination of two primers both consisting of a mixture of oligonucleotides (one complementary to the 5' end of VH segment and...
Topics
- Amino Acid Sequence
- Animals
- Antibodies, Antinuclear
- Antibody Affinity
- Base Sequence
- Clone Cells
- Genes, Immunoglobulin
- Immunoglobulin G
- Immunoglobulin M
- Immunoglobulin Variable Region
- Mice
- Mice, Inbred NZB
- Molecular Sequence Data
- Mutation
- Oligodeoxyribonucleotides
- Polymerase Chain Reaction
- Sequence Alignment