Article
Mutation screening of the TP53 gene by temporal temperature gradient gel electrophoresis.
Methods in molecular biology (Clifton, N.J.) - 1 Jan 2005
Sørlie Therese, Johnsen Hilde, Vu Phuong, Lind Guro Elisabeth, Lothe Ragnhild, Børresen-Dale Anne-Lise
Abstract excerpt
A protocol for detection of mutations in the TP53 gene using temporal temperature gradient gel electrophoresis (TTGE) is described. TTGE is a mutation detection technique that separates DNA fragments differing by single base pairs according to their melting properties in a denaturing gel. It is based on constant denaturing conditions in the gel combined with a temperature gradient during the electrophoretic run....
Topics
- DNA
- DNA Mutational Analysis
- Electrophoresis, Polyacrylamide Gel
- Genes, p53
- Humans
- Mutation
- Nucleic Acid Denaturation
- Temperature