Article
Electrophoresis for genotyping: temporal thermal gradient gel electrophoresis for profiling of oligonucleotide dissociation.
Nucleic acids research - 11 Jul 1995
Day I N, O'Dell S D, Cash I D, Humphries S E, Weavind G P
Abstract excerpt
Traditional use of an oligonucleotide probe to determine genotype depends on perfect base pairing to a single-stranded target which is stable to a higher temperature than when imperfect binding occurs due to a mismatch in the target sequence. Bound oligonucleotide is detected at a predetermined single temperature 'snapshot' of the melting profile, allowing the distinction of perfect from imperfect base pairing....
Topics
- Autoradiography
- Base Sequence
- Binding Sites
- Electrophoresis, Polyacrylamide Gel
- Exons
- Genotype
- Heterozygote
- Hot Temperature
- Humans
- Hydrogen-Ion Concentration
- Hyperlipoproteinemia Type II