Article
Selecting DNA fragments for mutation detection by temperature gradient gel electrophoresis: application to the p53 gene cDNA.
Electrophoresis - 1 Jul 1993
Ke S H, Kelly P J, Wartell R M, Hunter S, Varma V A
Abstract excerpt
Temperature gradient gel electrophoresis (TGGE) and related methods are widely employed to detect mutations in DNA fragments. DNA melting map calculations and GC clamps have been used to enhance the detection of mutations. While generally successful, these methods have not always revealed base ch...
Topics
- Base Sequence
- DNA
- Electrophoresis, Polyacrylamide Gel
- Genes, p53
- Humans
- Molecular Sequence Data
- Mutation
- Polymerase Chain Reaction
- Temperature