Article
Detection of mutations in exon 8 of TP53 by temperature gradient 96-capillary array electrophoresis.
BioTechniques - 1 Sept 2002
Kristensen A Torgunrud, Bjørheim J, Ekstrøm P O
Abstract excerpt
Various capillary electrophoresis applications have increasingly been utilized in mutation detection. Separation of two species is either based on secondary structure or differences in melting of DNA due to the mutation. Detection of the mutant is based on its mobility difference in the sieving matrix. We have adapted a regular 96-capillary sequencing instrument, the MegaBACE 1000, for mutation detection based on...
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