Article
A gene encoding a putative tyrosine phosphatase suppresses lethality of an N-end rule-dependent mutant.
Proceedings of the National Academy of Sciences of the United States of America - 15 Mar 1992
Ota I M, Varshavsky A
Abstract excerpt
The N-end rule relates the in vivo half-life of a protein to the identity of its N-terminal residue. In the yeast Saccharomyces cerevisiae, mutational inactivation of the N-end rule pathway is neither lethal nor phenotypically conspicuous. We have used a "synthetic lethal" screen to isolate a mut...
Topics
- Amino Acid Sequence
- Animals
- Base Sequence
- Blotting, Northern
- Blotting, Southern
- DNA, Fungal
- Drosophila
- Genes, Fungal
- Genes, Lethal
- Genotype
- Hot Temperature
- Humans
- Molecular Sequence Data
- Open Reading Frames
- Protein Tyrosine Phosphatases
- RNA, Fungal
- Saccharomyces cerevisiae
- Schizosaccharomyces