Article
The SAT1 flipper, an optimized tool for gene disruption in Candida albicans.
Gene - 27 Oct 2004
Reuss Oliver, Vik Ashild, Kolter Roberto, Morschhäuser Joachim
Abstract excerpt
The construction of Candida albicans mutants by targeted gene disruption usually depends on the use of nutritional markers for the selection of prototrophic transformants from auxotrophic host strains, but it is becoming increasingly evident that this strategy may cause difficulties in the interpretation of mutant phenotypes. Here, we describe a new method for inactivating both alleles of a target gene in C....
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