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Article

Sequential gene disruption in Candida albicans by FLP-mediated site-specific recombination.

Molecular microbiology - 1 May 1999

Morschhäuser J, Michel S, Staib P

Abstract excerpt

The genetic manipulation of the human fungal pathogen Candida albicans is difficult because of its diploid genome, the lack of a known sexual phase and its unusual codon usage. We devised a new method for sequential gene disruption in C. albicans that is based on the repeated use of the URA3 marker for selection of transformants and its subsequent deletion by FLP-mediated, site-specific recombination. A cassette...

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PMID: 10320577
DOI: 10.1046/j.1365-2958.1999.01393.x

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

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