Article
Polymorphism analysis within the HLA-A locus by universal oligonucleotide array.
Human mutation - 1 Nov 2004
Consolandi Clarissa, Frosini Andrea, Pera Cinzia, Ferrara Gian Battista, Bordoni Roberta, Castiglioni Bianca, Rizzi Ermanno, Mezzelani Alessandra, Bernardi Luigi Rossi, De Bellis Gianluca, Battaglia Cristina
Abstract excerpt
Human leukocyte antigen (HLA) class I genes present some of the most complex single nucleotide polymorphism (SNP) patterns in the human genome. HLA typing is therefore extremely challenging. In this article, we use the ligation detection reaction (LDR) combined with a universal array (UA) as a robust and efficient method to analyze SNPs within the HLA-A region that includes HLA-A alleles of interest for...
Topics
- Alleles
- Cluster Analysis
- DNA Mutational Analysis
- Exons
- Genotype
- HLA-A Antigens
- Humans
- Oligonucleotide Array Sequence Analysis
- Polymerase Chain Reaction
- Polymorphism, Single Nucleotide