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[Preliminary study on HLA-B genotyping by oligonucleotide chips].

Zhongguo shi yan xue ye xue za zhi - 1 Apr 2003

Lan Ke, Hu Shou-Wang, Zhang Fan, Wang Hui, Guan Wei, Ding Yu, Sun Ou-Jun, Wang Sheng-Qi

Abstract excerpt

HLA genes constitute a highly polymorphic multigene system. In the present study, HLA-B oligonucleotide chips were manufactured by using a set of sequence-specific oligonucleotide probes derived from polymorphic regions in exon 2 and exon 3 of HLA-B gene spotted by microarrayer onto the aldehyde modified glass slides. In addition, the sequenced HLA-B gene clones used as standard samples were amplified from exon 2...

Topics

Genotype
HLA-B Antigens
Humans
Oligonucleotide Array Sequence Analysis
Sequence Analysis, DNA

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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PMID: 12744741

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