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Article

Simultaneous genotyping of DRB1/3/4/5 loci by oligonucleotide microarray.

The Journal of molecular diagnostics : JMD - 1 Nov 2005

Bang-Ce Ye, Xiaohe Chu, Ye Fan, Songyang Li, Bincheng Yin, Peng Zuo

Abstract excerpt

Matching of the HLA antigens for donor-recipient in transplantation, disease predisposition or protection, population studies, and forensic testing requires accurate but simple typing methods. Here, we describe a DNA-based tissue-typing assay that determines the haplotype of the DRB1/3/4/5 loci in hy-bridization of oligonucleotide array after sample amplification. Using this multianalyte DNA hybridization system,...

Topics

Cell Line
DNA Probes
Exons
Fluorescent Dyes
Genetic Testing
Genotype
HLA-DR Antigens
HLA-DRB1 Chains
Humans
Introns
Nucleic Acid Hybridization
Oligonucleotide Array Sequence Analysis

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Polymerase Chain Reaction

Reproducibility of Results

Source

Open in PubMed Open full text

Identifiers

PMID: 16258157
DOI: 10.1016/S1525-1578(10)60592-2
PMCID: PMC1867558

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