Article
[Influence of DNA polymerases on mutation screening of denaturing high-performance liquid chromatography].
Ai zheng = Aizheng = Chinese journal of cancer - 1 Oct 2002
Liu Man-ran, Pan Kai-feng, Wang Yi, Lu You-yong
Abstract excerpt
BACKGROUND & OBJECTIVE: Recently, a sensitive and accurate technique called denaturing high-performance liquid chromatography (DHPLC) has been used in gene mutation screening, but several factors, especially DNA polymerase were discovered to affect the resolution of DHPLC technique. In order to find one or more suitable polymerases for DHPLC analysis, we designed this study to evaluate the influence of...
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