Restriction site generating-polymerase chain reaction (RG-PCR) for the probeless detection of hidden genetic variation: application to the study of some common cystic fibrosis mutations.

Abstract excerpt

In this report we describe the use of a DNA amplification technique in which modified primers introduce a base substitution adjacent to the codon of interest and create an artificial restriction site for the detection of mutations which do not produce or modify a naturally occurring restriction s...

Topics

• Base Sequence
• Cystic Fibrosis
• DNA, Single-Stranded
• Genetic Variation
• Humans
• Molecular Sequence Data
• Mutation
• Polymerase Chain Reaction
• Polymorphism, Restriction Fragment Length