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Proofreading genotyping assays mediated by high fidelity exo+ DNA polymerases.

Trends in biotechnology - 1 Feb 2005

Zhang Jia, Li Kai, Pardinas Jose R, Sommer Steve S, Yao Kai-Tai

Abstract excerpt

DNA polymerases with 3'-5' proofreading function mediate high fidelity DNA replication but their application for mutation detection was almost completely neglected before 1998. The obstacle facing the use of exo(+) polymerases for mutation detection could be overcome by primer-3'-termini modification, which has been tested using allele-specific primers with 3' labeling, 3' exonuclease-resistance and 3'...

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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PMID: 15661346
DOI: 10.1016/j.tibtech.2004.12.009

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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