Article
Multiplex short tandem repeat amplification of low template DNA samples with the addition of proofreading enzymes.
Journal of forensic sciences - 1 May 2011
Davis Carey P, Chelland Lynzee A, Pavlova Victoria R, Illescas María J, Brown Kelly L, Cruz Tracey Dawson
Abstract excerpt
With <100 pg of template DNA, routine short tandem repeat (STR) analysis often fails, resulting in no or partial profiles and increased stochastic effects. To overcome this, some have investigated preamplification methods that include the addition of proofreading enzymes to the PCR cocktail. This project sought to determine whether adding proofreading polymerases directly in the STR amplification mixture would...
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