Article
Isolation and characterization of ten mutator alleles of the mitochondrial DNA polymerase-encoding MIP1 gene from Saccharomyces cerevisiae.
Gene - 4 Jul 1995
Hu J P, Vanderstraeten S, Foury F
Abstract excerpt
Ten mutator alleles of MIP1, the gene encoding mitochondrial (mt) DNA polymerase, have been isolated after in vitro random mutagenesis. Five mutations causing a 100-400-fold increase in the frequency of erythromycin-resistant (ErR) mt mutants in yeast mapped to the 3'-5' exonuclease (Exo) domain,...
Topics
- Alleles
- Conserved Sequence
- DNA Polymerase I
- DNA, Mitochondrial
- DNA-Directed DNA Polymerase
- Exodeoxyribonuclease V
- Exodeoxyribonucleases
- Genes, Fungal
- Mitochondria
- Mutagenesis
- Phenotype
- Point Mutation
- Restriction Mapping
- Saccharomyces cerevisiae
- Saccharomyces cerevisiae Proteins