Article
Absence of point mutation of N-ras oncogene in bone marrow cells with aplastic anemia.
International journal of cell cloning - 1 Mar 1992
Shinohara K, Yujiri T, Kamei S, Ayame H, Tanaka M, Ando S, Tajiri M
Abstract excerpt
Point mutation of N-ras oncogene at codons 12, 13 and 61 was studied in the bone marrow cells of patients with typical aplastic anemia using the polymerase chain reaction method for DNA amplification and dot blot hybridization to synthetic oligonucleotide probes. Point mutation was observed in none of the 15 patients studied. These findings indicate either that the pathogenesis of typical aplastic anemia is...
Topics
- Anemia, Aplastic
- Base Sequence
- Blotting, Southern
- Bone Marrow
- DNA
- DNA Mutational Analysis
- Genes, ras
- Humans
- Molecular Sequence Data
- Mutation
- Nucleic Acid Hybridization