Article
Conserved tertiary base pairing ensures proper RNA folding and efficient assembly of the signal recognition particle Alu domain.
Nucleic acids research - 1 Jan 2004
Huck Laurent, Scherrer Anne, Terzi Lionel, Johnson Arthur E, Bernstein Harris D, Cusack Stephen, Weichenrieder Oliver, Strub Katharina
Abstract excerpt
Proper folding of the RNA is an essential step in the assembly of functional ribonucleoprotein complexes. We examined the role of conserved base pairs formed between two distant loops in the Alu portion of the mammalian signal recognition particle RNA (SRP RNA) in SRP assembly and functions. Mutations disrupting base pairing interfere with folding of the Alu portion of the SRP RNA as monitored by probing the RNA...
Topics
- Alu Elements
- Base Pairing
- Base Sequence
- Binding Sites
- Evolution, Molecular
- Molecular Sequence Data
- Mutation
- Nucleic Acid Conformation
- RNA, Small Cytoplasmic
- RNA-Binding Proteins
- Sequence Alignment
- Signal Recognition Particle