Article
Binding site of the M-domain of human protein SRP54 determined by systematic site-directed mutagenesis of signal recognition particle RNA.
Nucleic acids research - 15 Jan 1997
Gowda K, Chittenden K, Zwieb C
Abstract excerpt
The interaction of protein SRP54M from the human signal recognition particle with SRP RNA was studied by systematic site-directed mutagenesis of the RNA molecule. Protein binding sites were identified by the analysis of mutations that removed individual SRP RNA helices or disrupted helical sectio...
Topics
- Base Sequence
- Binding Sites
- Cloning, Molecular
- Electrophoresis, Polyacrylamide Gel
- Escherichia coli
- Gene Expression
- Humans
- Models, Molecular
- Molecular Sequence Data
- Mutagenesis, Site-Directed
- Mutation
- Nucleic Acid Conformation
- RNA
- RNA-Binding Proteins
- Recombinant Proteins
- Signal Recognition Particle