Article
Assembly of the human signal recognition particle (SRP): overlap of regions required for binding of protein SRP54 and assembly control.
RNA (New York, N.Y.) - 1 Oct 2001
Yin J, Yang C H, Zwieb C
Abstract excerpt
Assembly of the human signal recognition particle (SRP) entails the incorporation of protein SRP54, mediated by a protein SRP1 9-induced conformational change in SRP RNA. To localize the region that controls this crucial step in the assembly of human SRP RNA, four chimeras, Ch-1 to Ch-4, composed of portions of human and Methanococcus jannashii SRP RNAs, were generated by PCR site-directed mutagenesis from a...
Topics
- Base Sequence
- Binding Sites
- Chimera
- DNA Primers
- Humans
- Methanococcus
- Molecular Sequence Data
- Mutation
- Nucleic Acid Conformation
- Plasmids
- RNA, Bacterial
- Saccharomyces cerevisiae Proteins