Article
Interaction of protein SRP19 with signal recognition particle RNA lacking individual RNA-helices.
Nucleic acids research - 11 Jun 1991
Zwieb C
Abstract excerpt
Derivatives of human SRP-RNA were constructed by site-directed mutagenesis and tested for their ability to interact with protein SRP19. An RNA missing helix 6 barely interacts with SRP19, while the helix 8-deletion mutant retains much binding capability. A mutant RNA consisting just of helix 6 also binds the protein, but not as well as the unaltered molecule. SRP19 interacts to a full extent with the fourth...
Topics
- Base Sequence
- Binding Sites
- Electrophoresis, Polyacrylamide Gel
- Humans
- Molecular Sequence Data
- Mutagenesis, Site-Directed
- Mutation
- Nucleic Acid Conformation
- Plasmids
- RNA
- Ribonucleoproteins