Article
Stable, monomeric variants of lambda Cro obtained by insertion of a designed beta-hairpin sequence.
Science (New York, N.Y.) - 21 Dec 1990
Mossing M C, Sauer R T
Abstract excerpt
lambda Cro is a dimeric DNA binding protein. Random mutagenesis and a selection for Cro activity have been used to identify the contacts between Cro subunits that are crucial for maintenance of a stably folded structure. To obtain equivalent contacts in a monomeric system, a Cro variant was desig...
Topics
- Amino Acid Sequence
- Bacteriophage lambda
- Circular Dichroism
- DNA-Binding Proteins
- Escherichia coli
- Genetic Variation
- Macromolecular Substances
- Models, Molecular
- Molecular Sequence Data
- Mutagenesis
- Protein Conformation
- Repressor Proteins