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Article

Combinatorial redesign of the DNA binding specificity of a prokaryotic helix-turn-helix repressor.

Journal of bacteriology - 1 Jan 2003

Fromknecht Katja, Vogel Pia D, Wise John G

Abstract excerpt

Redesign of the bacteriophage 434 Cro repressor was accomplished by using an in vivo genetic screening system to identify new variants that specifically bound previously unrecognized DNA sequences. Site-directed, combinatorial mutagenesis of the 434 Cro helix-turn-helix (HTH) motif generated libraries of new variants which were screened for binding to new target sequences. Multiple mutations of 434 Cro that...

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Share to Bacteriophage lambda
Share to Combinatorial Chemistry Techniques

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Share to Escherichia coli

Share to Helix-Turn-Helix Motifs

Share to Operator Regions, Genetic

Share to Repressor Proteins

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Source

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Identifiers

PMID: 12511493
DOI: 10.1128/JB.185.2.475-481.2003
PMCID: PMC145310

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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