Article
Malignant transformation of human fibroblasts previously immortalized with 60Co gamma rays.
International journal of cancer - 20 Feb 1992
Mihara K, Bai L, Kano Y, Miyazaki M, Namba M
Abstract excerpt
Normal human fibroblasts obtained from a whole embryo were malignantly transformed with only 60Co gamma rays, but after extensive passage. The fibroblasts were exposed intermittently to 60Co gamma rays, 13 times, 2,800 rads in total, from the primary culture to the 40th passage level. At the 50th passage level the cells became morphologically changed, showing chromosomal abnormalities, and serial passages showed...
Topics
- Base Sequence
- Cell Transformation, Neoplastic
- Cells, Cultured
- Cobalt Radioisotopes
- Gamma Rays
- Genes, ras
- Humans
- In Vitro Techniques
- Karyotyping
- Molecular Sequence Data
- Mutation