Article
[Isolation and characterization of immortalized human fibroblasts].
Molekuliarnaia genetika, mikrobiologiia i virusologiia - 1 Jan 1997
Inozemtseva L S, Manuilova E S, Marshak M I, Nikolaeva N P, Gnedoĭ S N, Grivennikov I A
Abstract excerpt
A clone of immortalized human fibroblasts with an extended life span in culture was obtained after infection with a temperature-sensitive mutant (tsA 239) of SV40. In comparison with the parental cells, the clone exhibited an increase in the efficiency of plating, decreased doubling time, and serum dependence. No anchorage independence was observed. This means that the immortalized cells were not fully malignant,...
Topics
- Cell Line, Transformed
- Cell Transformation, Viral
- Cells, Cultured
- DNA, Viral
- Humans
- Karyotyping
- Phenotype
- Polymerase Chain Reaction
- Serial Passage
- Simian virus 40