Article
Isolation of dominant negative mutants and inhibitory antisense RNA sequences by expression selection of random DNA fragments.
Nucleic acids research - 25 Feb 1992
Holzmayer T A, Pestov D G, Roninson I B
Abstract excerpt
Selective inhibition of specific genes can be accomplished using genetic suppressor elements (GSEs) that encode antisense RNA, dominant negative mutant proteins, or other regulatory products. GSEs may correspond to partial sequences of target genes, usually identified by trial and error. We have used bacteriophage lambda as a model system to test a concept that biologically active GSEs may be generated by random...
Topics
- Bacterial Outer Membrane Proteins
- Bacteriophage lambda
- Base Sequence
- DNA, Viral
- Escherichia coli
- Genes, Dominant
- Genes, Suppressor
- Molecular Sequence Data
- Mutation
- Porins
- RNA, Antisense