Article
In vivo selection of spectinomycin-binding RNAs.
Nucleic acids research - 15 Dec 2002
Zimmerman Jeff M, Maher L James
Abstract excerpt
The folding of even short RNA molecules in a random library can produce a huge number of possible macromolecular structures. Using this principle, we have designed selections to seek non-coding RNA transcripts capable of interfering with specific macromolecules such as transcription factors in living bacterial cells. Here we show that such selections can uncover an unexpected class of RNAs. In the present case,...
Topics
- Anti-Bacterial Agents
- Base Sequence
- Binding Sites
- Binding, Competitive
- Drug Resistance, Microbial
- Escherichia coli
- Gene Library
- Molecular Sequence Data
- Mutation
- Nucleic Acid Conformation
- RNA, Bacterial
- Spectinomycin